3. a lab technician is preparing urea broth. it should be sterilized by filtration, he autoclaves it instead, just like he does for any other media. the high temperature of the autoclave causes the urea to break down. what color is the sterile urea broth when it comes out of the autoclave? but rstroedave tocave

The sterile urea broth after autoclaving, due to the high temperature causing the urea to break down, typically turns a yellow to orange color rather than its normal clear or orange-red color before sterilization. This is because autoclaving can degrade the urea and affect the pH indicator in the broth. Normally, sterile urea broth before testing appears clear and orange-red or yellowish, but improper sterilization by autoclaving can cause chemical changes that alter the color to yellow or orange due to breakdown products and pH shifts. In summary, if the urea broth is autoclaved instead of sterile- filtered, it will likely come out yellow or orange, not the expected color, due to urea degradation caused by the high heat of autoclaving.