It is generally recommended to take vitamin B12 supplements during the day rather than at night. This is because vitamin B12 boosts energy and can increase alertness, potentially interfering with sleep if taken at night. Experts advise taking B12 in the morning to align with the body's natural energy cycles and avoid sleep disturbances. However, if a doctor specifically advises taking it at night, those instructions should be followed. Vitamin B12 is water-soluble and best taken on an empty stomach or with a meal that facilitates absorption. Taking it in the morning can help reduce fatigue and support nerve function throughout the day. There is no strong clinical evidence banning nighttime intake, but many suggest avoiding it at night to prevent possible restlessness or insomnia due to its energizing effects.