eva green review

1 year ago 29
Nature

Eva Green is a green fluorescent nucleic acid dye that is used for several applications including quantitative polymerase chain reaction (qPCR) and melt curve analysis. It is a thermoresistant molecule that becomes highly fluorescent upon binding to double-stranded DNA. EvaGreen is essentially non-fluorescent by itself, but its DNA-binding properties allow target products to be effectively quantified at a significantly lower cost. EvaGreen is compatible with droplet-based digital PCR systems and is extremely stable both thermally and hydrolytically.

Positive:

  • EvaGreen is a green fluorescent nucleic acid dye that is useful for several applications including qPCR and melt curve analysis.
  • EvaGreen is thermoresistant and becomes highly fluorescent upon binding to double-stranded DNA.
  • EvaGreen is essentially non-fluorescent by itself, but its DNA-binding properties allow target products to be effectively quantified at a significantly lower cost.
  • EvaGreen is compatible with droplet-based digital PCR systems.
  • EvaGreen is extremely stable both thermally and hydrolytically.

Negative:

  • There is no negative information available about EvaGreen.

Ingredients or materials:

  • EvaGreen is a green fluorescent nucleic acid dye.
  • EvaGreen is a thermoresistant molecule.
  • EvaGreen is essentially non-fluorescent by itself.
  • EvaGreen becomes highly fluorescent upon binding to double-stranded DNA.