Lactulose generally takes about 24 to 48 hours to start working. It softens the stool by drawing water into the bowel to make passing stool easier. If constipation persists after 3 to 5 days of use, it is advised to consult a doctor. Lactulose is an osmotic laxative and is slower acting compared to some other types of laxatives which may work within hours, but lactulose typically needs a couple of days to have an effect.