what does the stage do on a microscope

1 year ago 85
Nature

The stage on a microscope is a crucial component that serves several functions:

  • Supporting the specimen: The stage is a flat platform that holds the specimen, typically a glass slide with the sample mounted on it. The specimen is secured in place by stage clips, which prevent it from moving during observation.

  • Enabling precise movement: In microscopes with a mechanical stage, the slide can be controlled using two knobs. One knob moves the slide left and right (X-axis), while the other moves it forward and backward (Y-axis). This feature allows for more precise positioning of the specimen, especially at higher magnifications, where manual movement can be challenging.

  • Facilitating scanning and exploration: The stages smooth translation and adjustable movement range make it easier to scan the specimen and explore different areas of interest. This is particularly useful when examining large or complex samples.

  • Enhancing imaging capabilities: Some microscope stages are designed to work with specific imaging techniques, such as micromanipulators with robotic arms for precise manipulation of specimens. Graduated or measuring stages may be equipped with hardware and/or software to allow for more accurate measurements.

  • Providing illumination: The stage is designed to be fully illuminated by the microscope's light source, ensuring optimal visibility of the specimen. This is achieved through either transmitted light (originating within or from below the stage) or reflected light (coming from the microscope or other light source above the stage).