Hyperpolarization is a process in which the membrane potential of a cell becomes more negative than its resting potential). In the context of an action potential, hyperpolarization occurs after repolarization and is the final stage of the action potential. During hyperpolarization, the cell is in a refractory period and is unable to generate subsequent action potentials). Hyperpolarization is caused by the efflux of cations, such as K+ ions, through the K+ channels embedded in the plasma membrane, or by the influx of anions, such as Cl- ions, through the Cl- channels. Hyperpolarization inhibits action potentials by increasing the stimulus required to generate them).