Interphase is the longest stage in the eukaryotic cell cycle, during which the cell acquires nutrients, grows, replicates its DNA, and prepares for cell division. Interphase is divided into three stages: G1, S, and G2. During G1, the cell grows and accumulates the building blocks of chromosomal DNA and the associated proteins as well as sufficient energy reserves to complete the task of replicating each chromosome in the nucleus. During S phase, DNA is duplicated into two sister chromatids, which are held together by a centromere. Finally, during G2, the cell grows and prepares for mitosis. Interphase is not a resting phase, but rather a metabolic phase of the cell in which it obtains nutrients and metabolizes them, grows, replicates its DNA in preparation for mitosis, and conducts other "normal" cell functions.