The best time to take magnesium glycinate depends on your specific health goals. For sleep support and relaxation, it is recommended to take magnesium glycinate 30 to 60 minutes before bedtime as it helps calm the nervous system and promote melatonin production. For managing anxiety, muscle cramps, migraine prevention, energy, or mood regulation, taking it in the morning or during the day may be more beneficial. If magnesium makes you feel sleepy during the day, then taking it at night is preferable. It is generally best to take magnesium glycinate with food to improve absorption and reduce potential stomach upset.