why is it important to start observation with 4x magnification

Starting observation with 4x magnification on a microscope is important because it provides a wider field of view which makes it easier to locate and navigate the specimen. The 4x lens allows viewing a larger area of the sample at once, helping to efficiently scan the slide and find regions of interest. It also offers a greater depth of field , which means more of the specimen stays in focus, facilitating clearer and more comprehensive observation. Additionally, using the lowest power objective initially reduces the risk of damaging the specimen, allows use of lower light intensity (which protects the sample and the microscope light source), and simplifies focusing. Once the area of interest is found with 4x magnification, the user can switch to higher magnifications for detailed examination with minimal adjustments needed due to parfocal lens design. In summary, starting with 4x magnification ensures efficient, safe, and effective initial scanning of the sample, setting up for more detailed observations at higher powers.