A skunk can spray up to 5 to 6 times in succession before its glands are depleted. After using its spray, it typically takes about 10 to 14 days for the skunk to replenish its supply. Because of this limited capacity, skunks usually only spray as a last resort when they feel truly threatened. In terms of frequency, wild skunks typically spray 1 to 2 times per year, or possibly once a month in higher stress environments, but this varies widely depending on the skunk's situation and environment.